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KMID : 0360220150560050771
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Journal of the Korean Ophthalmological Society
2015 Volume.56 No. 5 p.771 ~ p.775
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Effect of Nitric Oxide on the Permeability of Trabecular Meshwork Cell Monolayer
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Kim Hyun-Yeon
Kim Jae-Woo
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Abstract
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Purpose: To investigate the effects of nitric oxide (NO) on the permeability of cultured human trabecular meshwork cell (HTMC) monolayer.
Methods: HTMCs were cultured until confluency in the Transwell inner chamber and then exposed to 0, 10 or 100 μm S-nitroso-N-acetyl-DL-penicillamine (SNAP) and 0.5 mm L-NG-Nitroarginine methyl ester (L-NAME) for 24 hours. Permeabilities of carboxyfluorescein through the HTMC monolayer were measured using a spectrofluorometer after 2 hours in the outer chamber. Cellular viabilities and production of NO were assessed using 3-(4, 5 ?dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) and Griess assay, respectively.
Results: The cellular survival was not affected by 10 or 100 μm SNAP (p > 0.05) but NO production increased in a dose-dependent manner (p < 0.05). SNAP significantly increased the permeability of carboxyfluorescein through the HTMC monolayer in a dose-dependent manner compared with non-exposed control (p < 0.05). The endothelial NO synthase inhibitor L-NAME abolished SNAP-induced increase of the carboxyfluorescein permeability (p > 0.05).
Conclusions: NO increased the permeability of carboxyfluorescein through the HTMC monolayer in a dose-dependent manner. Thus, NO could increase trabecular outflow by increasing the permeability of trabecular cell layer in addition to trabeular messwork (TM) relaxation.
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KEYWORD
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Carboxyfluorescein, Nitric oxide, Permeability, Trabecular meshwork cells
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